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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: A modified protocol for the isolation, culture, and cryopreservation of rat embryonic neural stem cells
doi: 10.3892/etm.2020.9285
Figure Lengend Snippet: Macrodissection and microdissection processes used for brain collection. (A) The sacrificed pregnant rat was positioned on its back to expose the abdomen; the abdominal skin was then shaved and resterilized with povidone-iodine swabs. (B) The mesometrium and blood vessels were removed from the uterus (red line). (C) The uterus was then removed and rinsed. (D) High magnification image of C (arrow indicates malformed uterus). (E) While grasping one horn of the uterus in one hand using microforceps, the top of the uterus was carefully cut through with microscissors held in the other hand to open the uterus using a SZX16 light stereomicroscope. (F) The amniotic membrane was exposed. (G) The amniotic membrane was removed, and the placenta and embryo was exposed. (H) The embryo following removal of placenta and attached membranes. (I) The remaining embryos. (J) Straight microforceps (held in the non-dominant hand) were used to grasp the body of each embryo, while a pair of microscissors (held in the dominant hand) was used to cut the head of each embryo at the level of the cervical spinal cord. (K) The head was separated. (L) One pair of straight microforceps (held in the non-dominant hand) was used to steadily grasp each embryo head by placing the tip of the microforceps vertically along the caudal side of each ear, while the other pair of forceps (held in the dominant hand) was inserted into the nose (microforceps tip up) to peel off the skin with an expansion force through simultaneous bilateral tissue removal. (M) The cerebral hemispheres were harvested. (N) A pair of straight microforceps (held in the non-dominant hand) was used to steadily grasp the cerebral hemisphere, while the other pair (held in the dominant hand) was used to carefully peel off the pia mater and blood vessels. (O) A hemisphere with part of the pia mater and blood vessels. (P) A hemisphere was completely peeled off the pia mater and blood vessels.
Article Snippet: While grasping one horn of the uterus in one hand using microforceps (Shanghai Medical Instruments Co., Ltd.), the uterus was opened by careful incision of the top of the uterus using microscissors (Shanghai Medical Instruments Co., Ltd.) placed in the other hand, under a
Techniques: Laser Capture Microdissection